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Genetic Variations Affecting Complex Diseases
My research focuses on the identification and characterization of genetic variations that affect complex diseases. We take an integrated genomic approach to examine the alteration of mRNA/miRNA expression, copy number variation, single nucleotide polymorphism and DNA-protein interaction by primarily using microarray-based technologies for initial screening and using siRNA-mediated knockdown technologies for functional screening. Our goals are to identify genetic variations of clinical importance.
One of our ongoing research projects is to study the microRNA regulation of adipocyte differentiation. Using the combination of global gene array/miRNA array profiling, bioinformatics and siRNA knockdown, we have found that miR-17-92, a miRNA cluster that promotes cell proliferation in various cancers, can accelerate adipocyte differentiation by directly targeting a tumor suppressor, Rb2/p130.
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- Member, NIH Center for Scientific Review Special Emphasis Panel
- Member, International Mammalian Genome Society
- Member, American Society for Bone and Mineral Research
- Yan J, Jiao Y, Jian F, Stuard J, Donahue LR, Beamer WG, Li X, Roe BA, LeDoux MS, Weikuan G. Effects of carbonic anhydrase VIII deficiency on cerebellar gene expression profiles in the wdl mouse. Neuroscience Letters 2007; 413(3):196-201.
- Li X, Wang H, Touma E, Rousseau E, Quigg RJ, Ryaby JT. Genetic network and pathway analysis of differentially expressed proteins during critical cellular events in fracture repair. J Cell Biochem 2007; 100:527-543.
- Yan J, Jiao Y, Li X, Jiao F, Beamer WG, Rose CJ, Gu W. Evaluation of gene expression profiling in a mous model of L-gulonolactone oxidase gene deficienty. Genetics and Molecular Biology 2007; 30:322-329.
- Zhang Z, Yuan W, Sun L, Szeto FL, Wong KE, Li X, Kong J, Li YC. 1,25-dihydroxyvitamin D3 suppresses high glucose-induced NCP-1 expression in mesangial cells by targeting NF-†B. Kidney International (In Press).
- Jiao Y, Yan J, Jiao F, Yang HB, Donahue LR, Li X, Roe BA, Stuart J, Gu W. A single nucleotide mutation in Nppc is associated with a long bone abnormality in lbab mice. BMC Genetics 2007; 8:16.
- Schrump DS, Fischette MR, Nguyen DM, Zhao M, Li X, Kunst TF, Hancox A, Hong JA, Chen A, Pishchik V, Kruchin E, Wright JJ, Rosing DR, Sparreboon A, Figg WD, Steinber SM. Clinical and molecular responses in lung cancer patients receiving depsipeptide FK228 infusions. Clin Cancer Res (In Press).
- Li X, Wang H, Touma E, Qi Y, Rousseau E, Quigg RJ, Ryagy JT. TP508 accelerated fracture repair by promoting cell growth over cell death. Biochem Biophys Res Commun 2007; 264:187-93.
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